Journal: Scientific Reports

Article Title: In vivo imaging of kidney glomeruli transplanted into the anterior chamber of the mouse eye

doi: 10.1038/srep03872

Figure Lengend Snippet: (a) and (b) Transmission electron microscopy shows interdigitating podocyte foot processes. (c) Immunogold electron microscopy reveals conserved expression of podocalyxin in podocytes from transplanted glomeruli. Control sections stained with secondary antibody only were completely negative for gold particles.

Article Snippet: Briefly, Ultrathin sections were blocked with 1% ovalbumin in PBS for one hour, followed by incubation with affinity-purified goat antibody to podocalyxin (R&D, AF1556, 1:400) and an anti-goat 10 nm gold conjugate (1:50).

Techniques: Transmission Assay, Electron Microscopy, Expressing, Staining

Journal: Proteomics

Article Title: Identification, prioritization and evaluation of glycoproteins for aggressive prostate cancer using quantitative glycoproteomics and antibody-based assays on tissue specimens

doi: 10.1002/pmic.201200541

Figure Lengend Snippet: Standard Curve of ELISA assays for protein quantification of cartilage oligomeric matrix protein (COMP), periostin, membrane primary amine oxidase (VAP-1) and cathepsin L. A), dose-response curves for COMP. B), dose-response curve for periostin. C), dose-response curve for VAP-1. D), dose-response curve for cathepsin L.

Article Snippet: Materials Hydrazide resin and sodium periodate were from Bio-Rad (Hercules, CA); sequencing-grade trypsin and TMB reagent were from Promega (Madison, WI); PNGase F was from New England Biolabs (Ipswich, MA); C18 columns were from Waters (Milford, MA); Recombinant protein, capture and detection antibody of human cartilage oligomeric matrix protein (COMP), periostin, cathepsin L, clusterin and galectin-3 binding protein, streptavidin-HRP conjugates, ELISA plates and human VAP-1 Quantikine ELISA Kit were from R&D systems (Minneapolis, MN); All other chemicals were from Sigma-Aldrich (St. Louis, MO).

Techniques: Enzyme-linked Immunosorbent Assay